Food Microbe Tracker Help

Instructions for Searching by DNA Sequence

CONTENTS

Introduction

Search Food Microbe Tracker by DNA Sequence

INTRODUCTION

One unique function of the Food Microbe Tracker database is the ability to search the entire database for specific DNA sequences. Users of this database should possess a reasonable proficiency in performing and interpreting sequencing data.

Searching Food Microbe Tracker by DNA Sequence

1) From the main menu, click on “Search by DNA Sequence” (Figure 1).

Figure 1: Main menu - "Search by DNA Sequence"

2) You will be given options to choose the number of results (more will take longer), genus, species, sequence type, and DNA sequence (Figure 2).

Figure 2: Searching Food Microbe Tracker by DNA Sequence

3) To enter the sequence data in the dialog box, just cut from any text file and paste into the box . An example text file can be downloaded by clicking here.

4) Once all appropriate information is entered, click submit. The results will be displayed. For the example sequence, search against 16sRNA.

5) Isolates within the search results window are hyper-text linked to the all visible records within the database (demographics, PFGE type name, other genotypic and phenotypic characteristics) and links to BLAST format output are given.